Microbiology Lab Quiz 2 - Microbiology lab unit 2 quiz 8 Flashcards.

These games not only challenge your knowledge but also make l. Quiz yourself with questions and answers for microbiology lab quiz #2, so you can be ready for test day. Can simple staining techniques be used to identify more than the morphological characteristics of microorganisms?. Place the sample on the air drier for a short time, or until dry. Study with Quizlet and memorize flashcards containing terms like Dimorphism, Septate hyphae, Coencytic hyphae and more. what errors can occur during heat fixing? 1. Don't apply products such as cosmetics 5. -spreading often TMTC-streak = more isolate colonies and high concentration; may be more difficult to count due to confluent growth where streaking began-spreading: less confluent growth at lower conc. Quiz yourself with questions and answers for Microbiology Lab Exam 2, so you can be ready for test day. VT220 lecture-microbiology - Gram negative (-) Bacilli study guide. inncoulate saline with pure culture and incubate for 24 hours, separate into 2 tubes and add Methyl red to one (Red=mixed acid fermentatation) and Reagents A and B to the second (Red= 2,3 butanediol fermetation). Put 1 drop of lactophenol cotton blue on a slide (don't touch dropper to the slide!) 2. Study with Quizlet and memorize flashcards containing terms like inoculating a TTC tube, Wet mounts and hanging drop slide, evaporation or capillary action and more. A pure culture is usually derived from a m. The lower temperature in the fridge can slow the growth rate and impact the experiment. Study with Quizlet and memorize flashcards containing terms like What are the types of media you inoculated in the last lab?, When would you use a loop vs a needle?, What are some of the growth patterns that can be observed in a broth culture? and more. Flashcards; Learn; Test; Match; Q-Chat; Flashcards; Learn; Test; Match; Microbiology test 2. we know we are working with a live organism but we do our best to prevent any contamination (such as spillage, touching, etc. Flashcards; Learn; Test; Match; Q-Chat; Get a hint. Terms in this set (29) Study with Quizlet and memorize flashcards containing terms like Bacteria display ______ cell structure, having no membrane bound nucleus, Among pathogenic microorganisms, ___ are neither eukaryotic or prokaryotic, ___ of an element have varying number of neutrons, but the same number of protons in their nucleus and …. Quiz yourself with questions and answers for Microbiology Lab Quiz 2, so you can be ready for test day. Both are able to grow and isolate colonies, but streaking more effective at growing isolated colonies than spreading. High salt inhibits the growth of many bacteria. Simple staining technique involves the use of ___ dyes? 1. Terms in this set (176) Microbiology. iodine binds with starch to give a yellow halo. 4) does not affect organisms covered by solids and coverings. any medium (we used TSA) catalase reagent. local end dump jobs Microbio Chapter 6 Microbial Growth 93 terms. Microbiology - Lab Quiz: #2 (Labs 7 - 14) Define Tetrazolium salt (TTC). Microbiology 1 Narey Pre-Final & Final. Decolonized (acetone alcohol applied for 5 seconds) 4. Extracellular amylase indicated by a halo around bacterial growth where. Study with Quizlet and memorize flashcards containing terms like Culture medium, Microbes need, Aerobes and more. Study with Quizlet and memorize flashcards containing terms like Who developed Pure Culture Methods still used today?, What is the purpose of the Isolation Streak Lab, How does light affect the microscope images and more. Are you a trivia enthusiast looking to test your knowledge on the go? Look no further. pink broth, no bubble in tube = degradation of peptone; alkaline end products. Once they return to the intestinal tract, they mature into large adults who will mate. millville murder Describe the process of aseptic transfer from broth to agar slant. gram stain, acid-fast stain, endospore stain. Microbiology Lab - Quiz #2 Review. Phenol Red (PR) Glucose Test PR Lactose/PR Mannitol Broth Test. Are you looking to create a general knowledge quiz that will captivate and engage a wide range of participants? Crafting a quiz that appeals to a diverse audience requires careful. Floating in blood and kills foreign agents. Our mission is to improve educational access and learning for everyone. T/F With proper hand washing procedures, the surface of the skin can be sterilized and rendered free of bacteria. After incubation of a bacteriophage and its appropriate host, BLANK units can be counted on an agar plate. Most lab bacteria optimally grow at 37C. new tube of sterile medium is picked up, cap is removed, and neck of tube is flamed. determine the effects of normal serum and bactericidins. Notecards for Lab practical 2 Learn with flashcards, games, and. Describe a positive result in this experiment and describe a negative result. Go to 40x and use fine adjustment knob to focus 3. 0mL of water, Taking 1mL of water and distributing it to each microtube. Isolate that microbe from an infected subject and cultivate it artificially in the laboratory. Add basic carbol fuchsin dye and heat steam for 5 min. Quiz yourself with questions and answers for Microbiology Lab Test 2, so you can be ready for test day. Lab Safety Rules: Long pants; Gloves; Goggles if needed; Correct disposal (Biological waste goes in Biohazard bin) Lab coat; Tie up long hair, or loose dangling jewelry/sleeves. Explore quizzes and practice tests created by teachers and students or create one from your course material. 1 ml the sample in the final bottle was transfered to a plate and liquid agar was added. This video is a review of the material covered on quiz 2 for General Microbiology Lab (Biology 210L) at Orange Coast College (Costa Mesa, CA). Pour plates are made using dilute samples of bacteria and more. Be sure to spread mixture all around within the target area. The products of urease activity are. MR (+) organisms ____ the broth's pH. Cells +tive for endospores will stain. Microbiology -- Lab Quiz 2 -- Stains, Morphology, Arrangement. List five groupings of microorganisms according to their oxygen tolerance. Quiz yourself with questions and answers for Microbiology lab Quiz 2, so you can be ready for test day. quiz 4 microbiology lab chapter 10. _________ can grow in the presence of oxygen, but do not use it in metabolism. Add a small sample of bacteria and mix into water. Study with Quizlet and memorize flashcards containing terms like what to wear? 3 things, each experiment followed through to ? and lab manual up-to-date enter all ?, microscope care: microscope lenses must be cleaned with paper only. Water will flow in to the cell to achieve equilibrium. -Yellow: acid production or fermentation of the sugars in the slant or butt. Basic stains are positively charged and preferred because bacterial nucleic acids and cell wall components carry a negative chafe that attracts and binds the color. 5) not as effective against pigmented organisms. No unauthorized individuals will be admitted to the laboratory during. The fungal spores shown are produced asexually. - they contain a mixture of nutrients that can support the growth of a variety of microbial life. 5% NaCl, MSA, blood agar, MacConkey agar, and EMB agar) …. Lab Datasheets, Week 4 of 7- phenol red, MR-VP, Kirby-Bauer. - visually dominant pink will mask the blue and acid-fast bacteria will appear still hot pink. Focus your studying with a path. 1 - Flame loop from base to tip until it turns red hot. 4: Transferring the Inoculum into a Broth Tube; 2. WEEK 2 QUIZ - week 2 quiz with answers. -convert hydrogen peroxide into water and gaseous oxygen. Terms in this set (29) aseptic technique does what. selective medium for gram negative bacteria and differential. Study with Quizlet and memorize flashcards containing terms like Mycobacterium phlei (Acid Fast), Staphlylococcus epidermidis (Acid Fast), Mycobacterium phlei (Gram) and more. Antiseptics kill bacteria whereas disinfectants just inhibit the growth. list and discuss the functions of at least two chemicals involved in the innate immunity. Allied final (microbiology) 36 terms. VP (+) organisms are initially acidic, but because the 2,3-butanediol fermentation products are neutral, the pH at completion of the test is near _______. What must be done between each phase in a streak plate? Microbiology 325 Antibiotics 2. coli gram positive or gram negative?, Is staphylococcus aureus gram positive or negative?, Define general purpose growth media and more. Study with Quizlet and memorize flashcards containing terms like Using phase-contrast microscopy. Name: Score: 20 Multiple choice questions. Study with Quizlet and memorize flashcards containing terms like Gram Staining Procedure, Negative Staining Procedure, Negative Stain and more. Microbiology Lab Practical Exam Study Guide. These quizzes not only provide entertai. Study with Quizlet and memorize flashcards containing terms like What do you look through on a microscope to see the specimen?, What lenses are placed directly above the specimen for visuality?, What knobs moves the stage up and down? and more. Quiz yourself with questions and answers for Microbiology Lab Quizzes, so you can be ready for test day. After 24 h of incubation at 37C, how can you determine which is which? Click the card to flip 👆. , Staphylococcus aureus and Streptococcus pyogenes are ______ organisms. -The streak plate requires more skill. The target audience is primarily students preparing …. History of Microbiology: Germ Theory of Disease - flashcards. a chemical that destroys most or all pathogens on an inanimate object. - Used by the bacteria as an electron anchor. (water, air, ground) Media must be sterilized when working with microbes in the lab and we should avoid contamination. •Cells are maturing but not yet dividing. , so you can be ready for test day. shining c border collies What do these tubes indicate in PR broth? control is cloudy (valid test); negative result b/c no growth. Terms in this set (47) Equation for final dilution factor. Terms in this set (187) essential nutrients. Testing patients' blood for the presence or absence of antibodies against a particular pathogen. A small, single-celled organism that lacks a nucleus (instead, it has circular, covalently-closed DNA within the cytoplasm) and other organelles. 5 - Inoculate media with sample on loop. sterilize loop and rotate 90 degrees 4. MICROBIOLOGY - BISC 210 - FINAL. Promote the growth of the normal flora in the. aureus?, What happens to glucose tubes inoculated with B. The purpose of the videos and the quiz is to prepare you for performing this lab. What do we call circular double-stranded DNA that is able to self-replicate and is approximately 1-5% of the size of a bacterial chromosome?. Christmas trivia has gained immense popularity over the years due to its ability to bring people together and spark. 2- flame the loop to sterilize 3- clean surface and turn on the Bunsen burner 4- flame the mouth of the culture and replace the lid 5- obtain some bacteria by dipping the inoculation loop into the bacterial. -distorts size and shape of bacteria. Study with Quizlet and memorize flashcards containing terms like Differential medium, Selective medium, Selective (MacConkey Mac) and more. Terms in this set (46) Study with Quizlet and memorize flashcards containing terms like What are the 4 phases of the bacterial growth curve?, You will only see a typical bacterial growth curve when bacteria are grown in a, Which phase of growth occurs when the population of cells has stopped dividing due to lack of available nutrients? and more. On this plate the penicillin disc is at the top of the plate, with the positive control below on the left and the negative control below on the right. aseptic technique is used both when adding microbial samples to a plate and when moving colonies from one plate to another. Lab Quiz over exercise 6 and 8. received in the microbiology lab are mixed and the first step in identifying the causative agent so the streak plate is the first thing done •There are several types of methods to create the pure culture but the two we will be. the enzyme catalase converts hydrogen peroxide into water and oxygen. Quiz yourself with questions and answers for Microbiology - Lab Quiz 2, so you can be ready for test day. Study with Quizlet and memorize flashcards containing terms like Biochemical tests query one of two properties, Testing to see if a given bacterium secretes amylase by growing the microorganism on a nutrient agar plate containing starch. heat fixing for too long can cause the cells to become incinerated and not take up stain. Before lab: -Place all personal items in cubbies at front of lab. oakwood homes of powell one drop of oil immersion to slide on the stage 5. capsule remains unstained and forms a while ring around stained cell and background. 5, Which two microbial characteristics can be determined by doing a simple stain? a. cloudy- turbid, growth throughout. An organism that obtains organic food molecules by eating other organisms or their by-products. The 100x objective requires the use of: oil. -Red: negative or uninoculated. Terms in this set (24) Microbiology lessons often require incubation periods and active cultures. (When viruses are not viruses can only survive within a living host or. Place all media tubes in a test tube rack when not on use whether they are sterile or not. View Microbiology Lab Quiz 1 Answers. Study with Quizlet and memorize flashcards containing terms like Bacterial Culture Media, • A single microbial cell, supplied with the right nutrients and conditions, will multiply on the solid medium in a limited area to form a _____, a distinct mass of cells. Know what positive and negative results look like for each test. A speck of bacteria suspended in a broth medium that originated from a single cell. Mesophiles grow best at temperatures between. Microbiology lab quiz 2 study guide Lake-Sumter State College Learn with flashcards, games, and. Study Flashcards On Microbiology Lab Quiz 2 at Cram. Study with Quizlet and memorize flashcards containing terms like The pictured spores were most likely made by an Aspergillus mold. it dissolves and removes the primary stain from the cells. Allows to switch objectives w/o making major changes in focusing. Subjects Microbiology Lab Quiz #2. liquid preparations or suspensions of microbes, placed on a slide and cover slipped. Study with Quizlet and memorize flashcards containing terms like Taxonomy, Binomial system of nmenclature, Monera, protista, Fungi, Plantae, Animalia and more. If your microscope is equipped with an automatic stop, avoid using the stop; instead, use the coarse adjustment knob for bringing the image into focus. Solute greater on the inside of the cell. Flashcards; Learn; Test; Match; Q-Chat; Flashcards; Learn; Test; Match; When growing bacteria in the lab, after boiling to disolve the ingredients you sterilize the solution in a steam sterilization unit that is …. A "false foot" or temporary bulge of cytoplasm in the cell membrane used for feeding and movement in some protozoans. 3) spoangium engulfs forespore. Microbiology Lab - Week 2 Quiz (Aseptic Technique) What is aseptic technique? Click the card to flip 👆. Give today and help us reach more …. Incubate in 35C for 24 to 48hrs. bacteria and archaea shape - spirilla. What are the four different types of physical requirements when it comes to the needs of bacteria? Click the card to flip 👆. , The purpose of the little durham tube in the carbohydrate fermentation tubes is to detect: Gas production Acid …. how to pay sephora credit card online Holds the slide in place while on the microscope. There is a lot of overlap of what is covered in each Lab Quiz and each Lab Exam. Significance of coliforms and fecal coliforms in the context of food quality. Others show pigmentation or form a ring at the surface. Microbiology Banerjee Exam 3 - Respiration. with sterile loop pull some bacteria form are I to area II about 2 times and zigzag after without touching zone I 5. What is the purpose of vitamins as bacterial nutrients? Vitamins are essential coenzymes that act as a catalyst to speed up metabolic reactions. What is the importance of enzymes ? Click the card to flip 👆. The ingested prokaryotes remained alive inside their host and adopted special functions as organelles for a more complex cell. Finally, in some endospores, exoporium, a lipid-protein membrane, is formed outside the coat by the mother cell. Microbiology UTA 2460 - Lab Practical 2. Describe the genus Streptococcus. Mesophiles = growth at intermediate temps. What problems might that create in a microbiology lab or a surgical suite? and more. Microbiology Quizzes, Questions & Answers. both the Gram and the endospore. Microbiology (Lab #2) Microscope. Study with Quizlet and memorize flashcards containing terms like In a sucrose phenol red fermentation broth, sucrose is the sole fermentable nutrient. Revisit these Lab Quiz study guides when it is time to study for the Lab Exams and use the prompts to help you make questions for visual prompts in the Lab Exam PPT. Surface characteristics--> shiny, dull, rough, smooth. Study with Quizlet and memorize flashcards containing terms like What does complex media mean?, What does defined media mean?, Which media is more capable of supporting the widest range of bacteria? and more. stain slides w cystal violet for 60 secs. Study with Quizlet and memorize flashcards containing terms like A visible mass of cells or a _____ appears on a plate where cells were deposited, Color, size, shape, and texture of microbial growth are determined by the _____ _____ of the organism, but can be influenced by the _____, Recognizing different bacterial _____ _____ may be a useful step in the …. cell wall, cell membrane, protein synthesis, nucleic acid, synthesis, folic acid in cytoplasm, antibiotics inhibit the growth of microorganisms. The field of view is the: area of the slide that you can see. Minimize the potential of contamination 2. Bio 311 Microbiology Lab Quiz 2 (Lab 3 & 4) ) Lab 3 Objectives. To determine bacterial numbers, there are two standard methods used, what are they called? a. Quiz yourself with questions and answers for microbiology lab quiz 2, so you can be ready for test day. 119 terms Mbio 251 Practice questions. come to lab prepared- read lab BEFORE class 3. FDF = dilution factor of bottle * volume plated. what are two things that heat fixing achieves? -kills the bacteria. Study with Quizlet and memorize flashcards containing terms like The number of bacteria on the list of possible unknowns is _____ after the gram stain is determined. - use of muellar-hinton agar poured to a depth of 4 mm. -used to differentiate bacteria on the basis of fermentative or oxidative metabolism of carbohydrates-employs semi-solid medium in tubes containing carbohydrate under test (usually glucose) and a pH indicator-two tubes are inoculated and one is immediately sealed with mineral oil to produce anaerobic conditions. Study with Quizlet and memorize flashcards containing terms like supports the growth of a wide variety of microorganisms BUT distinguishes between two types. Microbiology Final - New Material. The ocular lens on the microscope can be rotated to different powers of magnification. technique used for testing antibiotic sensitivity. From Pierce College Lab Lecture Learn with flashcards, games, and more — for free. Terms in this set (161) What are the components of Endo Agar medium?-lactose-basic fuchsin-sodium sulfite. Pass the air dried slide through a flame three times to heat-fix the sample to the slide. -Any bubbling will appear instantly. blood group B can receive blood from. List the actions you must complete upon arrival and prior to departure in the microbiology lab. Answer: beer, ale, mead, wines, distilled alcoholic beverages, yeast bread. Study with Quizlet and memorize flashcards containing terms like Escherichia coli -- Cell morphology, Escherichia coli -- Cell arrangement, Escherichia coli -- Gram Stain Reaction and more. If no color change occurs after the addition of Nitrate A and B, add a small amount of zinc. (1) Obtain 2 small sterile plastic petri dishes with filter paper pads and m-Coli Blue 24 broth. Clinical Laboratory microbiology- HOSA. Adding VP reagents to the medium oxidizes. Hydrolytic reactions break down complex molecules by using. add grams iodine mordant to cover, set for 60 secs. Gas production is indicated by the agar lifting up or breaking. - This break down can be detected w/ Iodide. After a simple stain, bacterial cell morphology is determined under this magnification. Safranin dye is used as a counterstain in _________ stain (s). Lab Quiz 9&10 (unknown #3 media) 16 terms. Terms in this set (103) What is the purpose of acid-fast staining? Identifies organisms that are acid- fast, or organisms with a thick waxy layer containing mycolic acid. Do you find yourself struggling to keep up with conversations about current events or feeling left out during trivia nights? Don’t worry, you’re not alone. Do no waster water or natural gas. childe x ganyu broken horns how does temperature affect microbial growth? Click the card to flip 👆. laboratory must be left in orderly fashion: 1. Microbiology Lab Exam (STOP) 96 terms. You'll also encounter questions related to biochemical …. middle temperature loving (30- 37° C optimal) body temperature --> pathogens. Read on for some hilarious trivia questions that will make your b. another technique using microorganisms to extend the shelf life of food. Use a second slide to sweep the mixture across the first slide, making a color gradient. We suggest you take some informative microbiology trivia quizzes to enhance your knowledge. After you remove a cap from tubed medium, the cap should be. Immediately after, cover the specimen with cover glass utilizing a 45-degree angle to minimize the bubbles created or left under the cover glass, Large motile protozoa, small …. The greater thickness of the depression slide prevents one from being able to focus at the stop point. Study with Quizlet and memorize flashcards containing terms like differential medium, selective medium, MacConkey selective and more. burkes outlet credit card apply Metabolizes Glucose + gas + H2S (Black precipitate) Produces catalase. -When the reagent is oxidized by CYTOCHROME C, it it changes from colorless to a dark blue or purple compound. silkstockinglover Eosin-methylene Blue agar is selective for. Sample shown on left-hand side of blood agar plate. a simple stain that stains the background but leaves the bacteria unstained. These quizzes are a fun and interactive way to challenge yo. The number of CFUs that grew in and on the plate was 65. Millions of people take quizzes every day to learn more about themselves and to test their knowledge. a positive citrate test indicates that what enzyme is present. vitadone walmart You must prevent ______ and practice ________ techniques. How does autoclaving work? items are sterilized by steam at 121 Celsius and 15 1bs of pressure for 15 minutes or longer. - Known as the T-streak method, a T is written on the bottom so the compartments are divided into different sections to use as a template for streaking microbial cultures to isolate them. Some organisms flow on top of the medium and produces a type of surface membrane called a— PELLICLE 2. In microbiology, a pure culture is a laboratory culture that contains a single species of organism, according to Encyclopædia Britannica. Terms in this set (31) How do probiotics or prebiotics benefit the host? a. Study with Quizlet and memorize flashcards containing terms like 1. when atoms combine by sharing or transferring valence electrons. Simple stain uses 1 dye to increase contrast of cells. maytag bravo x Make sure all reagents are working correctly. B) it prevents movement of molecules out of the organism. Quiz yourself with questions and answers for Microbiology - Lab Quiz #2, so you can be ready for test day. elements that release energy during radioactive decay. , Saccharomyces cerevisiae substrates (what they ferment/grow on)? and …. The significance of the plasma membrane is that. resistant, intermediate, or sensitive. A mound of bacteria on a solid medium that originated with a single cell. 7)insert loop and zig-zag up the butt of the slant. Colony forming units (CFUs) Where colonies form. How do you want to study today? Flashcards. Terms in this set (55) What is the best definition of a mutation? random change in DNA sequence. Safranin (stains decolorized gram- cells). Identification of Unknown Bacteria Lab QUIZ. Microbiology 220 - Lab Quiz #2 study guide. 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Step 1: Label your two TSA plates and draw a T across the bottom of the agar plate. Terrific broth (TB) is a commonly used microbiological media. Test your knowledge of microbiology with this quiz! From defining pathogenic microorganisms to exploring the study of fungi and the protective forms produced by …. hartford craigslist cars Study with Quizlet and memorize flashcards containing terms like pure culture, mixed cultures, Morphology and more. att wireless fraud inquiry Allow the smears to air-dry completely before running the slides three times under Bunsen burner. flexible, spiral shape with internal flagella. View Test prep - Microbiology lab quiz 2 docx. The majority of members of Domain Bacteria are heterotrophs that derive carbon from organic compounds. What does the Decolorizing Solution, Ethanol, do to Gram-negative bacteria? Why?. -they way they ferment determines the difference in the tests MR and VP. Bacterial cultures to be gram stained must be 18-24 hours old for best …. The deciding factor in whether a bacterium will retain …. grow in a zone slightly below the surface or in the middle. Simply add about 2-3 drops of broth to the slide and then spread it around. Peel off tape and position between thumb and middle finger with adhesive side …. Standard media wiith standard thickness and pH, Mueller Hinton, 4 mm thickness, pH between 7. B antigens, Anti-A, can donate to B and AB, can receive B and O. – Once we grow microbes on the media, we can study their growth characteristics and other attributes (because now we have a bunch of the buggers to run experiments on) 23 g nutrient agar mix per 1 L (1000 mL) Previous Example: calculate the mass of nutrient agar powder to add for 100 mL of media 2 g. every microorganism has an optimum, a minimum, and a maximum growth temperature. Microbiology 20 - Lab Quiz 2 - Archana Mohan. if a yellow halo does not develop, the organism is able to. Quiz yourself with questions and answers for Microbiology Lab Quiz 2 (1), so you can be ready for test day. Lab Quiz Endospore Stain/Acid-Fast Stain (12&13) 27 terms. Microbiology Exam 2 Focus Guide. objects can be really close together and you can still tell them apart. If a bubble is present in durham tube. For class with Nia Madison for MCB2010L why are. Gram+: has thicker peptidoglycan cell walls Gram-: thinner cell wall Crystal violet is picked up by the cell. water only, regular soap, antibacterial soap, and hand sanitizer. , The purpose of flaming the loop is to_____. neither A nor B antigens and you have both anti-A and anti-B antibodies. Please choose all methods used to identify bacteria. - ATP is produced as a result of the transfer of electron through series of electron acceptors. Microbiology Lab Exam #2 Approved & Edited by ProProfs Editorial Team The editorial team at ProProfs Quizzes consists of a select group of subject experts, trivia writers, and quiz masters who have authored over 10,000 quizzes taken by …. , Bacterial endospores are:, During the Gram stain you use four solutions. Start at 10x and use course adjustment knob to focus 2. Microbiology Lab Quiz 2- labs 7-13 but not 12. Select all correct statements about the starch test results: A. Mix a loopful of broth culture with the nigrosin (no extra water is needed when the culture is taken from solid media as nigrosin contains a lot of water already. Organism Name, Media, Temp, Date, Lab #, Your Name Steps of Grahm Stain 3 Drops Crystal Violet for 1 min, Wash with distilled water, 3 drops Gram's Iodine for 1 min, Wash with distilled water, 2 drops alcohol wash immediatly, 3 Drops Safranin for 1. The charged portion of the chromophore is the auxochrome which allows the chromophore to act as a dye through ionic and covalent bonds between chromogen and cell. Terms in this set (19) In what ways can temperature affect microbial growth? Destroy proteins, affect membrane fluidity and destroy membrane potentials-this is what cells use to generate energy. A) Hold tube at 45°, B) Sterilize loop in flame, C) Pass tube over flame, D) Pick up bacteria with loop and touch on slide, E) Resterilize loop, F) Reclose tube, G) Reflame loop again, H) Let slide air dry, I) Pass slide over flame 4-5 times. in glucose, mannitol, and lactose tests if turned yellow. (requires use of acidic stain, with its negatively charged chromogen, doesn't penetrate cells because of the negative charge on the surface of bacteria). 2 most common food borne pathogens on chicken. Terms in this set (21) MSA - selects for salt. Micro205 Exam 1 Study Guide GCU. there is only one way to properly plate a microbial sample. Approved & Edited by ProProfs Editorial Team. what are the cardinal temps? Click the card to flip 👆. Study with Quizlet and memorize flashcards containing terms like Nutrient medium, Differential medium, Selective medium and more. * Used for analysis of water, food, food products, and sewage determination of Coliform contamination. Dement and Kleitman (1957) - Evaluation. proteins (gelatin) hydrolysis of macromolecules. Start studying Microbiology Lab Quiz #2 Study Guide. Chapter 17 Intro to Radiologic & Imaging Sciences & Patient Care. Try this amazing Microbiology Lab Exam #2 quiz which has been attempted 1598 times by avid quiz takers. 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