Microbiology Lab Quiz 2 - Microbiology Lab Quiz 1 Flashcards.

Safranin dye is used as a counterstain in _________ stain (s). boozefighters mc indiana compounds an organism cannot make itself and must acquire from its environment. detects gelatinase (an exoenzyme) that liquefies the solid medium. Explore quizzes and practice tests created by teachers and students or …. Autism spectrum disorder can affect the way a person interacts, communicates, and behaves. For class with Nia Madison for MCB2010L why are. The methods used to identify bacteria include microscopic appearance, colonial morphology, selective or indicating media, gram and other stains, biochemical reactions, gene probes, and antibody reactions. what are the cardinal temps? Click the card to flip 👆. Finals week is going to be my final week. any medium (we used TSA) catalase reagent. Bacteriophage (bacterial viruses) can replicate either by a lytic or lysogenic cycles. keilani bautista age Whether you are an educator, trainer, or business owner looking to test. 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Deposit culture in a line across area in 1st quadrant, then streak 1st quadrant. Exercises (6-11) *Important for practical *Unit 1 (Ch 1-3) Learn with flashcards, games, and more — for free. 3)Push the top slide in the opposite direction along the entire surface of the bottom slide. Study with Quizlet and memorize flashcards containing terms like tests the ability of MO's to ferment different carbohydrates into acid and may liberate gas, Durham tube apparatus - a large tube containing Phenol red broth, contains the test carbohydrate, and a phenol red pH indicator, and contains a small inverted test tube called the Durham tube. -The streak plate requires more skill. Flashcards; Learn; Test; Match; Q-Chat; Flashcards; Learn; Test; Match; Q-Chat; What is the importance of enzymes? Microbiology Pratical Day 2. -contains pH indicator phenol red (yellow < 6. Students shared 149 documents in this course. The chemical reaction failed to remove all of the oxygen in the jar. Study with Quizlet and memorize flashcards containing terms like Microbiological Media, Liquid media, Solid media and more. -Properly "drag" and "squiggle". If agent inhibits growth -> zone of inhibition-> minimum inhibitory concentration (MIC) - uses: 1. •Cells are maturing but not yet dividing. -convert water and oxygen into hydrogen peroxide. Microbiology Lab Quiz Two Multiple Choice. Once they return to the intestinal tract, they mature into large adults who will mate. Experiment - Test the question with an experiment. Chapter 3, Bacteria and Archaea Learn with flashcards, games, and more — for free. Lab Exam 2 Microscope Pictures. Exercise 3: Microbes in the Environment. Histoplasma capsulatum (Histoplasmosis) Coccidioides immitis (Coccidiomycosis) (valley fever) Cryptococcus neoformans (Cryptococcosis. Nemours normal blood coagulation values. work carefully/methodically- don't hurry 4. , The chemical reaction catalyzed by catalase is: and more. During incubation, agent diffuses from disk 2. Microbiology -- Lab Quiz 2 -- Stains, Morphology, Arrangement. What is the total magnification of a microscope which has a 20x ocular and you are using a low power objective? 200x. The exercises in this laboratory manual are designed to engage students in hand-on activities that reinforce their understanding of the microbial world. Terms in this set (41) Study with Quizlet and memorize flashcards containing terms like -Disinfect bench top -wash hands, -enrichment culture -'microbial ubiquity' -coined the term 'virus', growth in medium tailored to suit particular metabolic function and more. What is the purpose of phosphorus as a bacterial nutrient? Phosphorus is required for the production of nucleic acids and energy transport molecules such as ATP. Study with Quizlet and memorize flashcards containing terms like Autotrophs, Heterotrophs, Fastidious Bacteria and more. do no leave on the benchtop or leave old culture in incubator 3. 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The number of CFUs that grew in and on the plate was 65. aseptic technique is used both when adding microbial samples to a plate and when moving colonies from one plate to another. if a yellow halo does not develop, the organism is able to. - In a (-) result, it shows red only along the. Are you ready to put your mathematical skills to the test? Look no further than a fun and engaging math quiz. Study with Quizlet and memorize flashcards containing terms like What are the types of media you inoculated in the last lab?, When would you use a loop vs a needle?, What are some of the growth patterns that can be observed in a broth culture? and more. What does the Decolorizing Solution, Ethanol, do to Gram-negative bacteria? Why?. Terms in this set (47) Obligate aerobes. Casein, digest of animal tissue, beef extract, yeast extract, corn starch and sheeps blood. , The purpose of flaming the loop is to_____. Study with Quizlet and memorize flashcards containing terms like Escherichia coli -- Cell morphology, Escherichia coli -- Cell arrangement, Escherichia coli -- Gram Stain Reaction and more. the enzyme tested for is amylase. What do we call circular double-stranded DNA that is able to self-replicate and is approximately 1-5% of the size of a bacterial chromosome?. -distorts size and shape of bacteria. lab quiz #4 (10,11,12) 13 terms. What is the resolving power formula? wavelength of light in nm/ 2 * numerical aperture of the objective lens. These games not only provide entertainment but also offer a range. After growth, the plate is flooded with an iodine solution, which stains starch blue. Study with Quizlet and memorize flashcards containing terms like What is the best way to report an emergency when in the lab?, What is the emergency evacuation procedure?, Where is the Emergency Assembly Location? and more. Quiz yourself with questions and answers for Microbiology Lab Quiz #3 (Experiments 21, 22, 23, 24, 25, 28, 29), so you can be ready for test day. Study with Quizlet and memorize flashcards containing terms like To identify between gram positive and gram negative bacteria, Purple, Pink and more. -measure the diameter of each zone of inhibition in millimeters. 3) spoangium engulfs forespore. The lower temperature in the fridge can slow the growth rate and impact the experiment. The first focusing knob to use when focusing on a smear is the: course adjustment knob. Microbiology Final Study Guide. solid dilution technique; on agar plate; 3 sections, slowly physically dilute the bacteria by spreading it across the plate to see individual colonies. 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The 3rd step, decolorization with ethanol, is not able to wash the purple complex out of Gram-positive cells because they have thick cell walls. Terms in this set (45) when microbial growth is present in places where it is not suppose to be present -often used for infected tissue -in microbiology lab is is reffed to as contamination. Study with Quizlet and memorize flashcards containing terms like G+, G-, S. Used to assess a bacteria's ability to use malonate as its sole carbon source. a substance that enhances or strengthens the union between the dye and its substrate. Study with Quizlet and memorize flashcards containing terms like Differential medium, Selective medium, Selective (MacConkey Mac) and more. what color indicates a positive test for citrate utilization. Quiz yourself with questions and answers for microbiology lab quiz 2, so you can be ready for test day. 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What gives you total magnification? magnification of the objective lens x magnification of the ocular lens (10x) What is working distance? distance between objective lens and specimen (front surface of. Most lab bacteria optimally grow at 37C. A catalase test uses what reagent to determine a positive. Reisolate the agent from this subject. - This break down can be detected w/ Iodide. test for hydrogen sulfide gas production. Which 2 microbial characteristics can be determined by doing a simple stain? Shape and grouping pattern. Procedure: with a dilution scheme, 7 microtubes with 9. Its ribosomal components are similar, but somewhat smaller, than those of eukaryotes. c) can transfer DNA only to other F+ cells. , Saccharomyces cerevisiae substrates (what they ferment/grow on)? and …. what is the second possible reaction of TSI agar? if glucose and lactose and/or sucrose are fermented, sufficient acid will be produced to cause both the slant and but to remain yellow. Uses a reagent, tetra-methyl-p-phenylenediamine dihydrochloride. The 100x objective requires the use of: oil. new tube of sterile medium is picked up, cap is removed, and neck of tube is flamed. It is a nematode and a common intestinal parasite or helminth, especially in humans. Quiz yourself with questions and answers for Microbiology Lab Practical Exam 2, so you can be ready for test day. Applied as a mordant in a Gram Stain and form a complex with the Crystal Violet (CV-I Complex) within the cell. A positive result in an oxidase test will result in. Chapter 7 micro (exam 2) 19 terms. What is the chemical pathway for the Nitrate Test? -Nitrite (NO2 (+3) ) is reduced to Nitric Oxide (NO (+2)) then to Nitrous Oxide (N2O (+1)) and then finally to Molecular Nitrogen with no charge. Quiz yourself with questions and answers for Microbiology lab unit 2 quiz 9, so you can be ready for test day. Chapter 3 Knowledge Assessment: Tools of the Laboratory. Study with Quizlet and memorize flashcards containing terms like A visible mass of cells or a _____ appears on a plate where cells were deposited, Color, size, shape, and texture of microbial growth are determined by the _____ _____ of the organism, but can be influenced by the _____, Recognizing different bacterial _____ _____ may be a useful step in the …. both characteristics of bacteria and eukarya; simple cell structure. mixture in which nutrients are supplied for bacterial growth in a laboratory setting Agar- growth medium -colloidal polysaccharide (galactose polymer derived from seaweed) -no nutritional value -solidifying agent -unique properties (doesn't completely melt until 100 C and remains liquid until cooled to 45 C). Quizzes must be completed at least 2 hours prior to the start of your lab. 0% solid media usually contain agar concentration of allows for isolation of bacteria as a pure culture, and allows it to grow physical informative and useful ways. Study with Quizlet and memorize flashcards containing terms like Lipopolysaccharides are part of _____. -When the reagent is oxidized by CYTOCHROME C, it it changes from colorless to a dark blue or purple compound. iodine binds with starch to give a brown color. Dec 20, 2023 · Each quiz will feature multiple-choice questions that cover different aspects of microbiology lab techniques. Quiz 2 review Microbiology Lab Temperature & Growth of microbes Psychrophiles:-10C-20C Mesophiles:20C-40C Thermophiles:55C-75C Hyperthermophiles:Above 90C Extreme Thermophilic:Above 110C Bacterial used: -Pseudomonas fluorescens -Bacillus stearothermophilu. Learn the differences between gram positive and gram negative bacteria. With sterile loop pull some bacteria from area II to …. Terms in this set (160) Systemic fungal infections. microbiology-biomolecule structure and function. - want to determine if bacteria have a certain eco-enzyme (enzyme secreted by cell) to see if they can break down macromolecules and take in smaller components to metabolize. Study with Quizlet and memorize flashcards containing terms like Zinc is used as an inorganic catalyst in the test for_______. Place one loopful of nigrosin dye at the far end of a clean slide. Identify if one of the medias used in this lab (7. Microbiology - Lab Exam 2 - Everything else. dispose of all contaminated materials in appropriate contaminated discard containers 2. Study with Quizlet and memorize flashcards containing terms like 1. 3: Procedures and Results; 21: Appendicies. The greater thickness of the depression slide prevents one from being able to focus at the stop point. Bacteria that survive in the medium and utilize the citrate also convert NH3 and ammonium hydroxide, both of which tend to alkanize the agar. neither A nor B antigens and you have both anti-A and anti-B antibodies. -Any bubbling will appear instantly. Exercise 8 procedure steps 1-5. If your microscope is equipped with an automatic stop, avoid using the stop; instead, use the coarse adjustment knob for bringing the image into focus. Rinse with water until clear 7. These quizzes not only provide entertai. Surface characteristics--> shiny, dull, rough, smooth. Select all correct statements about the starch test results: A. replace bottles in rack on bench after use 4. Mannitol Salt Agar (MSA) This is a selective medium that favors the growth of staphylococci and for differentiation between pathogenic and non-pathogenic types. uses more than one stain (multiple steps) in order to differentiate organisms based on differences within a structure or the presence or absence of structures. working with microorganisms with the goal of preventing contamination of yourself and the microbial cultures you are using. cloudy- turbid, growth throughout. What contributes to the resistant properties? Spore coat. 7: Colony Morphology and Pigmentation; 2. The medical condition associated with pinworm infestation is known as pinworm infection or less precisely as oxyuriasis in reference to the family Oxyuridae. Terms in this set (39) Living cells are_____ charge, and simple staining uses a _____ charged dye to give cells color. Flashcards; Learn; Test; Match; Q-Chat; Flashcards; Learn; Test; Match; Microbiology test 2. Anyone who is at increased risk of acquiring. Microbiology 7 (nitrate and peptone deep) Quiz 1- Microbiology. In a lytic cycle, viral replication usually results in death and lysis of the host. Use a second slide to sweep the mixture across the first slide, making a color gradient. - Mechanical: cells with cilia physically sweep microbes outward, mucous membranes trap microbes, sneezes and coughs forcibly remove microbes. -Allowing proper spacing between colonies. Study with Quizlet and memorize flashcards containing terms like Know the parts of the microscope, Know the microscope parts with their functions, 400x and more. add hydrogen peroxide onto the plate and see if bubbles will form. Study with Quizlet and memorize flashcards containing terms like Is E. Guide for Quiz 2 Learn with flashcards, games, and more — for free. Foods that are made possible by both lactic acid and ethanol fermentation include: Answer: purple. walgreens mastercard credit score What defenses does the body have for fighting off pathogenic organisms on the skin? Click the card to flip 👆. Online quiz games have become increasingly popular in recent years, captivating players of all ages and providing a fun and interactive way to test knowledge and challenge the mind. proteins that help speed up chemical reactions in our bodies. The end result of the phenylalanine deaminase test is the same as the decarboxylase test, in that both tests aid in the catabolism of amino acids. A antigens, Anti- B, can donate to A and AB, can receive A and O. What is the chemical reaction that takes place during the Malonate Test? In one portion of the Krebs cycle, which requires the enzyme succinate dehydrogenase, the coenzyme FAD is reduced to FADH2. Microbiology Lab Exam #2 Approved & Edited by ProProfs Editorial Team The editorial team at ProProfs Quizzes consists of a select group of subject experts, trivia writers, and quiz masters who have authored over 10,000 quizzes taken by more than 100 million users. Don't apply products such as cosmetics 5. Changes in pH have little effect on protein structure and function. cereus was clear and blue black. Both are able to grow and isolate colonies, but streaking more effective at growing isolated colonies than spreading. Highly resistant to environmental conditions like heat, UV radiation. Study with Quizlet and memorize flashcards containing terms like obligate aerobes, facultative anaerobes, obligate anaerobes and more. Nonselective enriched differential. Study with Quizlet and memorize flashcards containing terms like inoculating a TTC tube, Wet mounts and hanging drop slide, evaporation or capillary action and more. View Test prep - Microbiology lab quiz 2 docx. Mar 18, 2024 · Microbiology Lab Exam #2 Approved & Edited by ProProfs Editorial Team The editorial team at ProProfs Quizzes consists of a select group of subject experts, trivia writers, and quiz masters who have authored over 10,000 quizzes taken by more than 100 million users. And others appear to clump in what is called— FLOCCULENT GROWTH 5. What are some bacteria morphology. Primary Stain (Gram Crystal Violet applied for 1 minute) 2. both acid-fast and endospores stains. These quizzes are a fun and interactive way to challenge yo. Take our customer service quiz to see how well you understand customer behaviors and preferences. List the directions to make a fungi plate: 1. what are advantages/disadvantages to no heat fixing? advantage: less distortion (no shrinkage of cells) disadvantage: shrinkage of cells. An unknown liquid sample was diluted by serial dilution and the final bottle had a dilution of 1/100,000. , allows one type of bacterium to grow but prevents the growth of others. Study with Quizlet and memorize flashcards containing terms like crystal violet dye, iodine, alcohol and more. Lab 2: Bacterial Isolation Learning Objectives Explain the importance of bacterial growth for the investigation of pathological microorganisms Use aseptic techniques Explain the concept of a single colony Perform plate-streaking techniques Use selective media for isolation purposes Techniques in Lab Colony screening Sterile technique Plate streaking Introduction: Bacterial Isolation Virtual. Exam 1 Study Guide - Microbiology. water only, regular soap, antibacterial soap, and hand sanitizer. rockhounding areas near me Cells +tive for endospores will stain. These addictive games not only provide entertainment but also offer num. A ____ color indicates an MR+ test, whereas a yellow color indicates an MR- test. all cells take up the purple dye in the first step of the staining procedure. star citizen bounty hunting guide Biosafety Levels in Microbiology Lab. Mannitol Salt agar is selective for. These types of bacteria would grow equally throughout a thioglycollate media tube. the bacterial strain oxidizes glucose. Add two loopfuls of bacteria and mix throughly with your loop. 0mL of water, Taking 1mL of water and distributing it to each microtube. multiple reagents are used and bacteria to react to the reagents. Do no waster water or natural gas. Lab: Final Practical Exam (Plus Quiz 5) 29 terms. Immediately after, cover the specimen with cover glass utilizing a 45-degree angle to minimize the bubbles created or left under the cover glass, Large motile protozoa, small …. Decolorized with acid alcohol for 5-10 sec 5. Most bacteria are colorless and provide minimal contrast Staining makes bacteria visibly observable To determine the morphology (form and structure), arrangements and size of bacteria. The pathogenic form of this microorganism can be ingested in food, and survive passage through the gastric acid barrier and invade the mucosa of the small and large intestine and produce toxins. What problems might that create in a microbiology lab or a surgical suite? and more. Microbiology: Examination of Eukaryotic Microbes: Lab 2. Learn the differences between simple staining and differential staining techniques. Study with Quizlet and memorize flashcards containing terms like How do probiotics or prebiotics benefit the host? a. when atoms combine by sharing or transferring valence electrons. starting at edge of plate streak back and forth in area one 3. 5: Transferring the Inoculum into a Petri Plate; 2. a positive citrate test shows that the bacteria can use citrate for it's _____ source. information from 1-4 3-5 3-6 3-7 3-8 Learn with flashcards, games, and more — for free. - If they make this enzyme will break down starch present in agar. Study with Quizlet and memorize flashcards containing terms like growth media, selective media, differential media and more. Study with Quizlet and memorize flashcards containing terms like The number of bacteria on the list of possible unknowns is _____ after the gram stain is determined. After 24 h of incubation at 37C, how can you determine which is which? Click the card to flip 👆. Study with Quizlet and memorize flashcards containing terms like What is the organisms involved in wine/beer?, What are the names of the two organisms involved with milk fermentations. Voges-Proskauer Test (Lab 24) Measures the production of the neutral end product acetoin by fermentation of glucose. craigslist estate sales louisville ky The editorial team at ProProfs Quizzes consists of a select group of subject experts, trivia writers, and quiz masters who have authored over 10,000 quizzes taken by more than 100 million users. Know the purpose of simple stains and what information you can learn from them. Tetramethyl-p-phenylenediamine is a chromogenic reducing agent. FDF = dilution factor of bottle * volume plated. What type of environment would you find archaea? Extreme heat, salt, and devoid of oxygen. amazon store card pay my bill blood group A can receive blood from. Our mission is to improve educational access and learning for everyone. Mix a loopful of broth culture with the nigrosin (no extra water is needed when the culture is taken from solid media as nigrosin contains a lot of water already. Place all media tubes in a test tube rack when not on use whether they are sterile or not. Study with Quizlet and memorize flashcards containing terms like True or False: As movement requires energy, metabolism can be defined as a controlled set of biochemical reactions that occur in only motile organisms in order to maintain life, True or False: Enzymes slow down chemical reactions to conserve energy, What is a cofactor? And give a general example (short answer) and more. What is the difference between antiseptics and disinfectants? Antiseptics are used on living tissue to decrease the number of microorganisms. Hold the inoculating loop or needle like a pencil in your dominant hand and relax. 5 - Inoculate media with sample on loop. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Jun 21, 2021 · The first focusing knob to use when focusing on a smear is the: course adjustment knob. Organisms are alive when viewed and can see motility. , Eosin methylene blue (EMB) media are selective because of __________________. These games not only challenge your knowledge but also make l. ny pets craigslist blood group B can receive blood from. Holds the slide in place while on the microscope. selective medium for gram negative bacteria and differential. a culture medium that contains protein digests, extracts like yeast extract of beef extract, or other ingredients that vary in chemical composition. Study with Quizlet and memorize flashcards containing terms like What does complex media mean?, What does defined media mean?, Which media is more capable of supporting the widest range of bacteria? and more. MICROBIOLOGY - BISC 210 - FINAL. This team includes our in-house seasoned quiz moderators and subject matter experts. Inoculate a susceptible healthy subject with the laboratory isolate and observe the same resultant disease. louis pasteur invented pasteurization which involves. Microbiology lab questions for lab quiz #2. - fermentation uses substrate-level phosphorylation. Stain can easily be washed from ______ _____ by rinsing with water. It is one of the most useful differential stains used in diagnostic microbiology. 1)Place a small drop of India Ink near one end of a slide. 2- flame the loop to sterilize 3- clean surface and turn on the Bunsen burner 4- flame the mouth of the culture and replace the lid 5- obtain some bacteria by dipping the inoculation loop into the bacterial. -they way they ferment determines the difference in the tests MR and VP. Know what positive and negative results look like for each test. What is the difference between antiseptics and disinfectants? Click the card to flip 👆. 3 - Collect bacteria sample on loop, holding tube on an angle to prevent contamination. You must prevent contamination and practice aseptic techniques by doing what 5 things. Prep for Quiz#1 on Lab Modules 1,2,3. Absence of color indicates amylase action. Study with Quizlet and memorize flashcards containing terms like a differential staining technique used to distinguish between bacterial cells that are capable of retaining a primary stain (acid-fast cells) and those that are not (non-acid-fast cells), useful for detecting and identifying certain bacterial pathogens that have unique cell wall structures, such as …. Microbiology Chapter 2 Pre-Lecture Assignment. General Microbiology Lab Midterm Exam. The decolorizing agent dissolves the outer lipopolysaccharide layer, and the CV-I washes out through the thin layer of peptidoglycan. oxidative phosphorylation vs fermentation. Subculturing prolongs the lifespan of the cells or micro. another technique using microorganisms to extend the shelf life of food. Significance of coliforms and fecal coliforms in the context of food quality. Terms in this set (19) In what ways can temperature affect microbial growth? Destroy proteins, affect membrane fluidity and destroy membrane potentials-this is what cells use to generate energy. Antiseptics are used on living tissue to decrease the number of microorganisms. Name: Score: 20 Multiple choice questions. A small, single-celled organism that lacks a nucleus (instead, it has circular, covalently-closed DNA within the cytoplasm) and other organelles. - Used by the bacteria as an electron anchor. Never hold a tube culture by its cap. How do bacteria propagate? Who invented the microscope? Check out our collection of online microbiology trivia quizzes to prepare for an upcoming test and …. 1) does not penetrate glass, plastic, cloth and other materials. camie utsushimi gif Study with Quizlet and memorize flashcards containing terms like In a sucrose phenol red fermentation broth, sucrose is the sole fermentable nutrient. A positive result in the oxidase test will appear as: A. Finally, in some endospores, exoporium, a lipid-protein membrane, is formed outside the coat by the mother cell. Prokaryotes (Part 1 and Part 2) 31 terms. * Used for analysis of water, food, food products, and sewage determination of Coliform contamination. A starch agar plate is streaked with Bacillus cereus and Pseudomonas aeroginosa and in incubated for 24hours. This free textbook is an OpenStax resource written to increase student access to high-quality, peer-reviewed learning materials. In microbiology, gram staining is an important test used because it can determine the presence of bacteria in a sample, as well as differentiate between the two distinct bacteria s. These are all the questions on the Lab Quiz 2 with the answers right underneath or next to the questions. Adhesins are: A) exotoxins and are involved in the first step of the infectious process. 3: Using a bacto-Incinerator to Sterilize the Inoculating Loop; 2. C) often found at the tip of pilli and are endotoxins. You have inoculated Salmonella enteriditis (which does not ferment lactose) and Escherichia coli (which does) onto different sectors on a plate of EMB agar. placement of a microbiological specimen from the source immediately onto growth medium. Standard antibiotic disc size with standard concentration of antibiotoc within disc 4. Study with Quizlet and memorize flashcards containing terms like Obligate Halophiles, Hypertonic, Isotonic and more. Orthodontics and Pediatrics NBDE 2. rinse off dye with water being careful not to damage the stained area. the use of a single stain to color a bacterial cell *split into 2 subcategories- basic (+) and negative (-). Aseptic technique prevents contamination of: the culture plate, the environment, and yourself. b) The production of NH3 from the peptones which lead to pseudo pink colouration can be inhibited by maintaining anaerobic conditions, thereby limiting oxidation reactions during the fermentation. Exercise 9/10: Gram Stain (Reagent chemistry with two different types of bacterial cell …. Terms in this set (24) Microbiology lessons often require incubation periods and active cultures. Flashcards; Learn; Test; Match; Q-Chat; Flashcards; Microbiology Exam 2 (REVIEW BASED) 82 terms. Study Flashcards On Microbiology Lab Prelab Quiz #2 at Cram. What is the Gram charge of this bacteria? Mycobacterium smegmatis. 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How do you want to study today? Flashcards. microbiology: lab 2 pre lab quiz flashcards. Contains components that will either enhance the growth of specific organisms or inhibit growth of specific organisms. There is a lot of overlap of what is covered in each Lab Quiz and each Lab Exam. Bi 234 - Microbiology Lab Quiz 2. Learn with flashcards, games, and more — for free. Put 1 drop of lactophenol cotton blue on a slide (don't touch dropper to the slide!) 2. - pyruvate must be converted into a fermentation product to allow NAD+ to become NADH, can also produce a wide. In order to determine the identity of the bacterium, you have performed the following tests: 1. - Once we grow microbes on the media, we can study their growth characteristics and other attributes (because now we have a bunch of the buggers to run experiments on) 23 g nutrient agar mix per 1 L (1000 mL) Previous Example: calculate the mass of nutrient agar powder to add for 100 mL of media 2 g. The range of zone diameters, expressed in millimeters, between the sensitive and resistant zone diameters where individual diffrences between patients and bacterial strains may cause one particular combination to be resistant while another combination might prove to be sensitive. coli gram positive or gram negative?, Is staphylococcus aureus gram positive or negative?, Define general purpose growth media and more. received in the microbiology lab are mixed and the first step in identifying the causative agent so the streak plate is the first thing done •There are several types of methods to create the pure culture but the two we will be. The growth rate is influenced by temperature, nutrient availability, pH, oxygen levels, etc. Metabolizes Glucose + gas + H2S (Black precipitate) Produces catalase. Gram-negative bacteria are mostly located in the human _____. the enzyme catalase converts hydrogen peroxide into water and oxygen. 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Gram negative bacteria stain a. anionic, which means that, an ionization of the stain, the chromogen portion exhibits a negative charge and therefore has strong affinity for the positive constituents of the cell. positive: cracks or bubbles in agar. Lab Quiz 1 Microbiology, Microbiology Lecture Quiz 1, Microbiology Lab Quiz 2, Micro Lecture Quiz 2, Disease Study Guide Exam 1 Ch1-3. Selective, Enriched Media, Differential. 1) uses electrons instead of lights, 2) uses magnets to focus, and 3) uses a heavy metal or gold for staining (this allows the specimen to live up to 5 min) 1) whole objects (able to see), 2) 3 dimensional view, 3) intact cells and viruses, and 4) magnification 1,000 - 10,000x. - Take sample from culture, dilute it, then plate samples to grow. GCU BIO205 Quiz 2 Review (ch 4) 29 terms. How many reagents are used to perform a gram stain? Four. Primary stain, carbolfuchsin dye, binds to bacteria, HOT PINK. 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Chapter 4 lecture (micro) 55 terms. gram negative enterics (eosin-methylene blue inhibits gram +) Microbiology lab quiz #2. Pink/red; Bacterial cultures to be gram stained must be 18-24 hours old for best staining results. Standard concentration of organism, 0. Quiz yourself with questions and answers for Microbiology Lab: Quiz 2 ( Simple stain, Negative stain, & Gram Stain), so you can be ready for test day. It consists of a light source, a filter which allows only a single wavelength of light to pass through, the sample tube containing the bacterial suspension, and a photocell that compares the. What is a complex medium ? Click the card to flip 👆. Microbiology Lab Final Study Guide WCU. Chapter 17 Intro to Radiologic & Imaging Sciences & Patient Care. Course G282L/MCB2289L Section 03 Introduction to Microbiology Lab - Online - 2018. Cultures that only contain 1 microbial Species (used in labs) Click the card to flip 👆. If you like to take quizzes, you are not alone. 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The ocular lens on the microscope can be rotated to different powers of magnification. -used to differentiate bacteria on the basis of fermentative or oxidative metabolism of carbohydrates-employs semi-solid medium in tubes containing carbohydrate under test (usually glucose) and a pH indicator-two tubes are inoculated and one is immediately sealed with mineral oil to produce anaerobic conditions. kills bacteria causing them to adhere to the slide. Microbiology lab quiz 2 study guide Lake-Sumter State College Learn with flashcards, games, and. Quiz yourself with questions and answers for Microbiology Lab Quiz 2 Exercise 11 Protein Metabolism, so you can be ready for test day. multiply the number of colonies by the dilution then multiply the answer by 4. 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Floating in blood and kills foreign agents. to teach and develop those skills that will make the student competent and confident while working in the microbiology laboratory. Phenol Red (PR) Glucose Test PR Lactose/PR Mannitol Broth Test. Incubate in 35C for 24 to 48hrs. Malonate, when added to the media, is similar enough to succinate to.